high production of il-18 by dendritic cells induced by sera from patients with primary antibody deficiency
نویسندگان
چکیده
predominantly antibody deficiencies are a category of primary immunodeficiency diseases, which consist of several rare disorders such as common variable immunodeficiency (cvid) and x-linked agammaglobulinemia (xla). we evaluated the effects of cvid and xla patients’ sera as a source of microenviromental factors on maturation and function of monocyte-derived dcs. blood was collected from 10 cvid and 5 xla patients before immunoglobulin replacement therapy and also from 8 healthy volunteers in order to obtain necessary sera for this study. monocyte derived dcs were generated from blood cells obtained from healthy volunteers in the presence of gm-csf, il-4 and 10% serum concentrations from cases and controls. immature dcs were incubated with monocyte conditioned medium (mcm) and tnf-α in order to generate mature dcs. interleukin 18 (il-18) production by cd40l-activated mature dcs was measured after 24 hours of culture in vitro. il-18 production by dcs generated in the presence of cvid and xla patients’ sera were 6.75±2.59 and 7.08±1.75 ng/ml, respectively, which were significantly higher than normal serum conditioned dcs (3.55±0.68) ng/ml. these results suggest that the sera of patients with predominantly antibody deficiencies may contain soluble factor(s) that can induce a significant increase in il-18 production by dcs.
منابع مشابه
IL-18-based combinatorial adjuvants promote the intranodal production of CCL19 by NK cells and dendritic cells of cancer patients
The effective accumulation and interaction of mature dendritic cells (DCs) and naïve T cells within lymph nodes (LNs), which are driven by the CCR7-CCL19/CCL21 chemokine axis, are critical for the induction of adaptive T-cell immunity. Human natural killer (NK) cells activated by interleukin (IL)-18 exhibit a unique 'helper' activity in promoting productive DC-T cell interactions, inducing DC m...
متن کاملSynergistic effects of IL-4 and IL-18 on IL-12-dependent IFN-gamma production by dendritic cells.
Mouse splenic dendritic cells (DCs) produce IFN-gamma in response to IL-12. In the present study, we analyzed effects of Th1 and Th2 cytokines on IFN-gamma production by DCs. IL-18 produced by DCs and macrophages acts in an autocrine manner and augments IL-12-induced IFN-gamma production by DCs as also observed in T and NK cells. Surprisingly, IL-4, a Th2 cytokine, also acts synergistically wit...
متن کاملSynergistic Effects of IL-4 and IL-18 on IL-12-Dependent IFN-g Production by Dendritic Cells
متن کامل
Butyrate increases IL-23 production by stimulated dendritic cells.
The gut microbiota is essential for the maintenance of intestinal immune homeostasis and is responsible for breaking down dietary fiber into short-chain fatty acids (SCFAs). Butyrate, the most abundant bioactive SCFA in the gut, is a histone deacetylase inhibitor (HDACi), a class of drug that has potent immunomodulatory properties. This characteristic of butyrate, along with our previous discov...
متن کاملLipopolysaccharide-induced suppression of airway Th2 responses does not require IL-12 production by dendritic cells.
The prevalence of atopic asthma, a Th2-dependent disease, is reaching epidemic proportions partly due to improved hygiene in industrialized countries. There is an inverse correlation between the level of environmental endotoxin exposure and the prevalence of atopic sensitization. As dendritic cells (DC) have been implicated in causing sensitization to inhaled Ag, we studied the effect of endoto...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
iranian journal of allergy, asthma and immunologyجلد ۶، شماره ۲، صفحات ۵۹-۶۵
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023